COMPARATIVE ULTRASTRUCTURAL AND MOLECULAR-CELLULAR CHARACTERISTICS OF THE STATE OF THE UROTISSUE BARRIER OF THE URINARY BLADDER IN WOMEN WITH NORMAL CONDITIONS, RECURRENT BACTERIAL AND LATE RADIATION CYSTITIS (PILOT STUDY)
DOI: 10.22138/2500-0918-2025-22-1-17-27
B.A. Berdichevsky¹, V.A. Shidin¹, D.A. Uchaev², A.F. Arslanov¹,
A.R. Gonyaev³, M.A. Korabelnikov4, G.V. Zubik¹
¹Federal State Budgetary Educational Institution of Higher Education Tyumen State Medical University of the Ministry of Health of the Russian Federation, Tyumen, Russian Federation;
²Department of Electron Microscopy of the Scientific and Educational Center «Nanotechnology» Federal State Autonomous Educational Institution of Higher Education «South Ural State University», Chelyabinsk, Russian Federation;
³Limited Liability Company «MOTHER AND CHILD TYUMEN»,
Tyumen, Russian Federation;
4State Autonomous Institution of Healthcare of the Tyumen Region "Multidisciplinary Clinical Medical Center «Medical City», Tyumen, Russian Federation
Abstract. Aim. Abstract Chronic inflammation of the bladder is polyetiological, but has a common pathogenetic platform associated with a violation of the ureteral barrier (UB) facilitating the penetration of urinary toxins to various levels of cellular wall formations. The aim of the study was to investigate the possibility of clinical assessment of the ultrastructural and molecular-cellular state of the UB of the bladder by scanning electron microscopic (SEM) and positron emission tomography (PET/CT) with 11C-choline. Materials and methods. Comparative studies of the results of SEM and PET/CT of the UB state were conducted in 2 women of working age before and 12 months after external beam radiotherapy (EBRT) for cervical cancer at stage TIIN0M. Results. As a result of the analysis of the SEM and PET/CT image of the epithelial layer of the bladder of a patient with no urological history before EBRT, its densely packed structure with a standardized 11C-choline uptake level (SUVmax) of 5.44 U was revealed. After 12 months of the relapse-free period of the underlying disease, with two clinical and laboratory episodes of manifestations of PLC, deformation of the urothelium with the formation of intercellular channels was detected, coinciding with an increase in SUVmax by 57.9%. The results of the analysis of the SEM and PET/CT image of the superficial layer of the bladder of a patient with a history of recurrent cystitis (RC) before EBRT made it possible to visualize a slight expansion of the intercellular space accompanied by an increase in SUVmax by 12.5% above the indicator in an uncomplicated urological history. After 12 months, against the background of a relapse-free period of the underlying disease, with four clinical and laboratory episodes of manifestations of PLC, further expansion of the intercellular space was observed with a 5-fold increase in SUVmax relative to the PLC indicator without concomitant RC. Conclusion. The PET/CT indicator of the standardized level of 11C-choline uptake (SUVmax) in the bladder wall can reflect the ultrastructural state of the ureteral barrier, which requires further study and clarification.
Keywords: scanning electron microscopy, PET/CT, ureteral barrier, cystitis
There is no conflict of interest.
Contact details of the corresponding author:
Boris A. Berdichevsky
doktor_bba@mail.ru.
Received: 01.11.2024
For citation: Berdichevsky B.A., Shidin V.A., Uchaev D.A., Arslanov A.F., Gonyaev A.R., Korabelnikov M.A., Zubik G.V. Comparative ultrastructural and molecular-cellular characteristics of the state of the urotissue barrier of the urinary bladder in women with normal conditions, recurrent bacterial and late radiation cystitis (pilot study). [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2025, Vol. 22, no. 1, pp. 17–27. DOI: 10.22138/2500-0918-2025-22-1-17-27 (In Russ)
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Authors
Boris A. Berdichevsky
Federal State Budgetary Educational Institution of Higher Education Tyumen State Medical University of the Ministry of Health of the Russian Federation
MD, Professor, Professor of the Department of Surgery and Urology with a Course in Endoscopy
Tyumen, Russian Federation
doktor_bba@mail.ru
Vladimir A. Shidin
Federal State Budgetary Educational Institution of Higher Education Tyumen State Medical University of the Ministry of Health of the Russian Federation
Head of the Department of Histology with Embryology, MD, Associate Professor
Tyumen, Russian Federation
vshidin@mail.ru
Daniil A. Uchaev
Department of Electron Microscopy of the Scientific and Educational Center «Nanotechnology» Federal State Autonomous Educational Institution of Higher Education «South Ural State University»
Research fellow
Chelyabinsk, Russian Federation
uchaevda@susu.ru
Artem R. Gonyaev
Limited Liability Company «MOTHER AND CHILD TYUMEN»
Head of the Urology Department
Tyumen, Russian Federation
a.gonyaev25@yandex.ru
Aidar F. Arslanov
Federal State Budgetary Educational Institution of Higher Education Tyumen State Medical University of the Ministry of Health of the Russian Federation
Postgraduate student of the Department of Histology with Embryology
Tyumen, Russian Federation
Mikhail A. Korabelnikov
State Autonomous Institution of Healthcare of the Tyumen Region "Multidisciplinary Clinical Medical Center «Medical City»
Radiologist
Tyumen, Russian Federation
kma_doc@mail
Grigory V. Zubik
Federal State Budgetary Educational Institution of Higher Education Tyumen State Medical University of the Ministry of Health of the Russian Federation
Student of the Faculty of Medicine and Prevention
Tyumen, Russian Federation
grisha.zubik@mail.ru