Aging and dynamics of herodiagnostic biomarkers in human

I.V. Gavrilov^1,2, V.N. Meshchaninov^1,2, D.L. Shcherbakov^1,2, T.Iu. Verzhbitskaia1,

N.S. Manakova^1,2, N.M. Cherepanova1, E.M. Varlashov1, E.D. Reshetnikov^1,2

1Institute of Medical Cell Technologies, Yekaterinburg, Russian Federation;

2Ural State Medical University, Yekaterinburg, Russian Federation

Abstract. The aim of the study was to identify and compare the information content of the main biochemical, cellular-hematological and functional-psychological biomarkers, reflecting the processes of physiological and accelerated (pathological) aging of the patient’s body for use in diagnosing their biological age. Materials and methods. A clinical randomized parallel controlled study was carried out, in which the age-related dynamics of biochemical, including lipid peroxidation and antioxidant activity (LPO and AOA), cellular hematological, including stem pluripotent hematopoietic cells CD34 +, and functional and psychological parameters of the body of practically healthy people under physiological aging and accelerated polymorbid pathology, recorded by the state of their biological age (BA). A total of 5296 patients were examined voluntarily: 3395 males and 1901 females. The examined patients had a calendar age (CA) from 18 to 93 years, were divided by sex and health status (practically healthy patients and patients with polymorbid pathology with accelerated aging — in a 1: 1 ratio). Results. Biochemical, cellular-hematological and functional-psychological indicators of the organism to varying degrees reflected the rate of aging and the duration of the forthcoming life span. Functional and psychological indicators most optimally reflected the rate of aging of the body, since they turned out to be the most calendar-age-dependent. Biochemical and cellular-hematological indicators did not reveal such a high age dependence, to a greater extent being more stable and conservative, reflecting the stability of the body’s homeostasis and, apparently, the probable life expectancy. Judging by the results of the correlation analysis, the biological age calculated using additional derivatives from the measured base values, especially if functional and psychological indicators are used for this, most accurately reflects the rate of aging, in contrast to the bio-age calculated using only basic indicators of direct measurement.

Keywords: aging, biological age, markers of aging, cellular and hematological parameters, stem cells

There is no conflict of interest.

Contact details of the corresponding author:

Iliya V. Gavrilov

given18@yandex.ru

Received 26.11.2020

For citation:

Gavrilov I.V., Meshchaninov V.N., Shcherbakov D.L., Verzhbitskaia T.Yu., Manakova N.S., Cherepanova N.M., Varlashov E.M., Reshetnikov E.D. Aging and dynamics of herodiagnostic biomarkers in human. http://vestnikural.ru/article/1149 [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 4, pp. 272–284. DOI: 10.22138/2500-0918-2020-17-4-272-284 (In Russ)

REFERENCES

1.      Anisimov V.N. Molecular and physiological mechanisms of aging in 2 vol., 2nd ed. revised and add. S.-Pb. The science. 2008 (in Russ)
2.      López-Otín C., Blasco M. A., Partridge L. et al. The hallmarks of aging. // Cell. 2013. Vol. 153. No. 6. P.1194-1217. doi: 10.1016 / j.cell.2013.05.039.
3.      Gavrilov L.A., Gavrilova N.S. Is aging a disease? The point of view of biodemographers // Advances in gerontology. 2017.Vol. 30. No. 6.P. 841-842. (in Russ)
4.      Golubev A.G. Is aging a disease? The point of view of a biogerontologist: old age ≠ disease // Advances in gerontology. 2017. T. 30. No. 6. P. 845-847. (in Russ)
5.      Moskalev A.A. Is aging a disease? The point of view of genetics // Advances in gerontology. 2017.Vol. 30. No. 6.P. 843-844. (in Russ)
6.      Miakotnykh V.S. Is aging a disease? The point of view of the geriatrician // Advances in gerontology. 2017.Vol. 30. No. 6.P. 848-850. (in Russ)
7.      Gavrilov I.V., Meshchaninov V.N. Assessment of the rate of aging in animals and humans using biochemical, hematological and functional indicators // Health, demography, ecology of the Finno-Ugric peoples. 2017. No. 3. S. 70-75. (in Russ)
8.      Gavrilov I.V., Meshchaninov V.N., Shcherbakov D.L. et al. Screening of functional, biochemical and cellular-hematological parameters of the body as markers of the aging process in humans // Bulletin of the Ural Medical Academic Science. 2018.Vol. 15. No. 5.P. 691-703. (in Russ)
9.      Meshchaninov V.N., Shcherbakov D.L., Lukash V.A. Metabolism of cellular structures during aging and stress. Monograph // Yekaterinburg, 2017. (in Russ)
10.    Meshchaninov V.N., Tkachenko E.L., Gavrilov I.V. and others. Medical diagnostic and therapeutic cell-metabolic technologies in preventive gerontology and geriatrics — the results of work for 10 years // Bulletin of the Ural Medical Academic Science. 2016.T. 59. No. 4.P. 76-86. (in Russ)
11.    Gavrilov I.V., Meshchaninov V.N. Various methodological techniques of digitalization in measuring human bio-age // New information technologies in education and science. 2020. No. 3. S. 28-31. (in Russ)
12. Justice J.N., Ferrucci L., Newman A.B. et al. A framework for selection of blood-based biomarkers for geroscience-guided clinical trials: report from the TAME Biomarkers Workgroup // Geroscience. 2018. Vol. 40. No. 5-6. P.419-436. doi: 10.1007 / s11357-018-0042-y
13. Dauber K., Becker D., Odendahl M. et al. Enumeration of viable CD34 (+) cells by flow cytometry in blood, bone marrow and cord blood: results of a study of the novel BD ™ stem cell enumeration kit. Cytotherapy. 2011; 2: 449-458.
14. Gavrilov I.V., Meshchaninov V.N., Leontiev S.L., Sazonov S.V. Computer program «Bioage polinom» // Certificate of registration of the computer program RU 2012613817. Application No. 2012611835 dated 15.03.2018.
15. Gavrilov I.V., Meshchaninov V.N., Tkachenko E.L., Lukash V.A., et al. Method for determining biological age in men // Invention patent RU 2617313 C1, 04.24.2017. Application No. 2016129302 dated July 18, 2016. (in Russ)
16. Gavrilov I.V., Meshchaninov V.N., Tkachenko E.L., Lukash V.A., et al. Method for determining biological age in women // Invention patent RU 2617801 C1, 04.24.2017. Application No. 2016129651 dated 07.19.2016. (in Russ)

Authors

Iliya V. Gavrilov

Institute of Medical Cell Technologies, Center providing specialized types of medical care

Ph.D., Associate Professor, Senior Researcher, Laboratory of Anti-Aging Technologies

22a, Karl Marks st., Yekaterinburg, Russian Federation, 620026

Ural State Medical University

Associate Professor of the Department of Biochemistry

3, st. Repin, Yekaterinburg, Russian Federation, 620014

given18@yandex.ru

Victor N. Meshchaninov

Institute of Medical Cell Technologies, Center providing specialized types of medical care

Doctor of Medical Sciences, Professor, Chief Researcher, Head of the Laboratory of Anti-Aging Technologies

22a, Karl Marks st., Yekaterinburg, Russian Federation, 620026

Ural State Medical University

Head of the Department of Biochemistry

3, st. Repin, Yekaterinburg, Russian Federation, 620014

mv-02@yandex.ru

Denis L. Shcherbakov

Institute of Medical Cell Technologies, Center providing specialized types of medical care

Ph.D., Senior Researcher, Laboratory of Anti-Aging Technologies

22a, Karl Marks st., Yekaterinburg, Russian Federation, 620026

Ural State Medical University

Assistant at the Department of Biochemistry

3, st. Repin, Yekaterinburg, Russian Federation, 620014

cdcom2@yandex.ru

Tatyana Yu. Verzhbitskaia

Institute of Medical Cell Technologies, Center providing specialized types of medical care

Candidate of Medical Sciences, Leading Researcher, Laboratory of Cell Therapy of Oncohematological Diseases

22a, Karl Marks st., Yekaterinburg, Russian Federation, 620026

imct@celltechnologies.ru

Nadezhda. S. Manakova

Institute of Medical Cell Technologies, Center providing specialized types of medical care

Doctor of clinical laboratory diagnostics of the laboratory of anti-aging technologies

22a, Karl Marks st., Yekaterinburg, Russian Federation, 620026

Ural State Medical University

Senior Laboratory Assistant, Department of Biochemistry

3, st. Repin, Yekaterinburg, Russian Federation, 620014

imct@celltechnologies.ru

Natalia. M. Cherepanova

Institute of Medical Cell Technologies, Center providing specialized types of medical care

Doctor-geriatrician of the laboratory of anti-aging technologies

22a, Karl Marks st., Yekaterinburg, Russian Federation, 620026

imct@celltechnologies.ru

Evgenii M. Varlashov

Institute of Medical Cell Technologies, Center providing specialized types of medical care

Research assistant laboratory of anti-aging technologies

22a, Karl Marks st., Yekaterinburg, Russian Federation, 620026

varlash@mail.ru

Evgeniy. D. Reshetnikov

Institute of Medical Cell Technologies, Center providing specialized types of medical care

Laboratory Technician, Anti-Aging Technology Laboratory

22a, Karl Marks st., Yekaterinburg, Russian Federation, 620026

Ural State Medical University

Senior Laboratory Assistant, Department of Biochemistry

3, st. Repin, Yekaterinburg, Russian Federation, 620014

imct@celltechnologies.ru