IMPACT OF (SODIUM) AMINOPHTHALHYDRAZIDE ON INSULIN+ AND PDX1+ RAT LIVER CELLS IN EXPERIMENTAL MODEL OF DIABETES MELLITUS TYPE I

DOI: 10.22138/2500-0918-2021-18-2-68-77

M.B. Baikenova ¹^,², K.V. Sokolova ², I.F. Gette ², I.G. Danilova ²

¹ Ural Federal University named after the first President of Russia B.N. Yeltsin,
Yekaterinburg, Russian Federation;
² Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Science,
Yekaterinburg, Russian Federation

Abstract. Aim: to evaluate quantity and functional activity of insulin+ hepatic cells in response to modulation of macrophage functional activity in rats in the modelling of diabetes mellitus type 1. Materials and methods: experiment was carried out on male Wistar rats, which were divided into 3 groups: intact, DM and DM+APH. To model DM alloxan in the dose of 170 mg/kg was injected intraperitoneally, the dose of APH was 2 mg/kg intramuscularly (a total of 20 injections within 30 days). Biochemical, enzyme immunoassay, immunohistochemical and statistical research methods were used. Results: сompare to the DM, in DM+APH group the increasing numbers of insulin+ hepatocytes and insulin+ sinusoidal cells were detected. At the same time, the total number of sinusoidal cells and Pdx1+ liver cells increase in both DM and DM+APH groups. It was found, that the content of insulin in the cytoplasm of insulin+ hepatocytes significantly higher in the DM+APH group, compare to intact and diabetic animals, which indicates the impact of APH at the growth of functional activity of insulin+ hepatocytes. An increase in the expression of insulin in the hepatic cells at the modulation of macrophage activity by APH may contribute to the correction of hyperglycemia in diabetic rats. Conclusion: introduction of APH leads to an increase in the number of insulin+ hepatocytes and their insulin-producing function, as well as insulin+ sinusoidal cells in animals with experimental diabetes. An increase in the total number of sinusoidal hepatic cells when the APH was administered in animals with alloxan-induces DM was revealed. At the same time, the action of APH enhances the expression of Pdx1 — a factor of differentiation for insulin-producing cells in the liver of diabetic animals.

Keywords: diabetes mellitus, liver, immunomodulators, aminophthalhydrazide, insulin+-cells, pdx1+-cells

There is no conflict of interest.
Contact details of the corresponding author:
Madina B. Baikenova
m.b.baikenova@urfu.ru
Received 12.05.2021
For citation:
Baikenova M.B., Sokolova K.V., Gette I.F., Danilova I.G. Impact of (Sodium) Aminophthalhydrazide on Insulin+ and Pdx1+ Rat Liver Cells in Experimental Model of Diabetes Mellitus Type. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2021, Vol. 18, no. 2, pp. 68–77. DOI: 10.22138/2500-0918-2021-18-2-68-77 (In Russ)

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Authors
Madina B. Baikenova
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences
Junior researcher
106 Pervomaiskaya str. Yekaterinburg Russian Federation 620049
Ural Federal University named after the first President of Russia B.N. Yeltsin
PhD student
19 Mira str. Yekaterinburg Russian Federation 620002
m.b.baikenova@urfu.ru

Ksenia V. Sokolova
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences
Junior researcher
106 Pervomaiskaya str. Yekaterinburg Russian Federation 620049
kssocolova@bk.ru

Irina F. Gette
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences
Candidate of Science in Biology, Senior Researcher
106 Pervomaiskaya str. Yekaterinburg Russian Federation 620049
i.goette@yandex.ru

Irina G. Danilova
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences
Doctor of Biological Science, Associate Professor, Head of the Laboratory of Morphology and Biochemistry
106 Pervomaiskaya str. Yekaterinburg Russian Federation 620049
ig-danilova@yandex.ru

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)