THE EFFECT OF AEROBIC EXERCISE ON THE FATTY ACIDS CONTENT AND THEIR COMPLEXES IN THE WHITE ADIPOSE TISSUE OF RATS CONSUMING HIGH CALORIE DIETS

DOI: 10.22138/2500-0918-2019-16-3-384-400

I.Yu. Yakimovich, M.Yu. Kotlovsky, S.V. Gusakova, V.V. Ivanov, V.N. Vasilyev, A.M. Dygai, I.V. Dolgalev, A.V. Panimaskina, L.Yu. Kotlovskaya

Siberian State Medical University, Tomsk, Russian Federation;
Goldberg Research Institute of Pharmacology and Regenerative Medicine, Тomsk, Russian Federation

Abstract. Objective: to study the effect of aerobic exercise on the fatty acids percentage and their complexes in the adipose tissue of rats of different localization consuming high calorie diets.Materials and methods. The study was conducted on male white rats (Wistar). The animals were divided into two groups and followed a high calorie diet (with a 32% fat share in the total calorie content). One group of animals did low-intensity aerobic exercise (6 weeks), another one had no regular activity. Mesenteric, retroperitoneal, epididymal and subcutaneous inguinal adipose tissues were isolated. The fatty acids (FA) were determined by a chromato-mass spectrometer (Agilent, USA). 24 FA and 14 integrative complexes were studied.Results. Physical activity decreased the unsaturated (UFA), polyunsaturated FA (PUFA) and unsaturation index in mesenteric tissue. Saturated FA (SFA) were increased. The value of the unsaturation index was decreased. The content of polyunsaturated fatty acids (PUFA) was decreased. An increase in the content of palmitic (C16: 0) SFA was noted. Toward the palmitic (C16: 0) SFA, its ratio with the oleic (C18: 1) monounsaturated fatty acid (MUFA) was changed. FA-substrates of energy was decreased in retroperitoneal adipose tissue and increased in membranes substrates. A decrease in the amount of MUFA was noted. The fatty acid-substrates content of vitamin F and their ratio with MUFA and oleic (C18: 1) ω9 UFA were increased. The value of the ω6 UFA amount was increased due to linoleic (C18: 2) UFA.The amount of ω3 EFA was decreased in mesenteric and retroperitoneal adipose tissue. The ratio ω3 / ω6 was shifted towards ω6 UFA. The content of stearic (С18: 0) SFA was increased.Conclusion. Aerobic exercise and high calorie diet modified the percentage of FA in white adipose tissue of rats. The most significant effect was recorded for FA in mesenteric and retroperitoneal tissue, and the smallest for FA in epididymal and subcutaneous tissue.

Keywords: fatty acids, fatty acid complexes, adipose tissue, aerobic exercise, diet

Received 14.05.2019

For citation: Yakimovich I.Yu., Kotlovsky M.Yu., Gusakova S.V., Ivanov V.V., Vasilyev V.N., Dygai A.M., Dolgalev I.V., Panimaskina A.V., Kotlovskaya L.Yu. The Effect of Aerobic Exercise on the Fatty Acids Content and their Complexes in the White Adipose Tissue of Rats Consuming High Calorie Diets. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2019, Vol. 16, no. 3, pp. 384-400. DOI: 10.22138/2500-0918-2019-16-3-384-400 (In Russ)

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Authors
Inessa Yu. Yakimovich
Siberian State Medical University
Cand. Sci. (Med.), Associate Professor, Head of department of Hygiene
Russian Federation, 634050, Tomsk, Moscow tract, 2 
iness2501@yandex.ru

Mikhail Yu. Kotlovsky
Goldberg Research Institute of Pharmacology and Regenerative Medicine of Tomsk National Research Medical Center
Dr. Sci. (Med.), Research Fellow
Russian Federation, 634028, Tomsk, Lenin Prospect, 3 
m.u.kotlovskiy11443@mail.ru

Svetlana V. Gusakova
Siberian State Medical University
Dr. Sci. (Med.), Head of the Department of Biophysics and Functional Diagnostics, Dean of the Faculty of Medical and Biological Sciences
Russian Federation, 634050, Tomsk, Moscow tract, 2 
gusacova@yandex.ru

Vladimir V. Ivanov
Siberian State Medical University
Cand. Sci. (Biol.), Associate Professor, Associate Professor of the Department of Biochemistry and Molecular Biology with a course of clinical laboratory diagnostics
Russian Federation, 634050, Tomsk, Moscow tract, 2 
ivanovvv1953@gmail.com

Vladimir N. Vasilyev
Siberian State Medical University
Dr. Sci. (Biol.), Professor, Professor of the Department of Physical Culture and Health
Russian Federation, 634050, Tomsk, Moscow tract, 2 
vas11@yandex.ru

Aleksandr M. Digai
Goldberg Research Institute of Pharmacology and Regenerative Medicine of Tomsk National Research Medical Center Honored Worker of Science of the Russian Federation
Academician of the Russian Academy of Sciences, Dr. Sci.(Med.), Professor, Secretary of the Life Science Section of the Russian Academy of Science Medical Department,
Academic Advisor of Goldberg Research Institute of Pharmacology and Regenerative Medicine of Tomsk National Research Medical Center
Russian Federation, 634028, Tomsk, Lenin Prospect, 3
nii@pharmso.ru

Igor V. Dolgalev
Siberian State Medical University
Dr. Sci. (Med.), Professor, Head of department of Faculty Therapy with a course of clinical pharmacology
Russian Federation, 634050, Tomsk, Moscow tract, 2 
div65@mail.ru

Anna V. Panimaskina
Siberian State Medical University
Assistant of the Department of Faculty Therapy with a course of clinical pharmacology
doctorpav@yandex.ru

Larisa Yu. Kotlovskaya
Goldberg Research Institute of Pharmacology and Regenerative Medicine of Tomsk National Research Medical Center
Research assistant
Russian Federation, 634028, Tomsk, Lenin Prospect, 3 
lars.ktl@gmail.com