Influence of background lympopolic on the reactions of systemic immunity during short-term total cooling
DOI: 10.22138/2500-0918-2019-16-2-170-176
V.P. Patrakeeva, V.A. Schtaborov
Institute of Environmental Physiology of Federal Center for Integrated Arctic Research Russian Academy of Sciences, Arkhangelsk, Russian Federation
Abstract: Aim to determine the peculiarities of the formation of immune reactions in response to short-term overall cooling during lymphopenia. Materials and methods. A complex of immunological examination of 91 women of working age living in Arkhangelsk before and immediately after the general cooling in the cold chamber (for 5 minutes at –25°С) was carried out. The determination of hemogram parameters was performed on a XS-1000i hematology analyzer. On lymphocytes, markers of maturity, activation, differentiation, apoptosis were determined. In smears stained by Romanovsky-Giemsa, monocytogram, lymphocytogram, and neutrogram were determined. In the blood plasma, the content of catecholamines (adrenaline, norepinephrine) was evaluated by ELISA. Results and its discussion. It was established that the background immune status of people with lymphopenia is characterized by a lower content of monocytes, without changing their functional activity. An increase in ATP concentration in lymphocytes during lymphopenia indicates a higher level of demand for their energy supply, which is, in our opinion, a compensatory response. Changes in the microvasculature are involved in the complex of compensatory reactions, as evidenced by the increase in the content of norepinephrine. In lymphopenia, in response to general cooling, a reaction is recorded from the side of neutrophil granulocytes, which is accompanied by an increase in their number in circulation. Given the short period of exposure to the cold factor, such a reaction is likely due to the activation of the redistribution of cells from the marginal pool to the circulating one, and this is unlikely due to the release of cells from the depot, i.e. circulatory system of the lungs. A feature of the reaction to general cooling in lymphopenia, against the background of a compensatory reaction from the blood cells and catecholamines is to maintain the overall level of lymphocyte concentration due to the release of immature forms of T-cells (CD3-) from the depot against the background of a decrease in the blood noradrenaline level. Under these conditions, a noticeable decrease in the T-cell activation content is observed, mainly with the membrane receptor for transferrin and CD4 +.
Keywords: lymphopeniya, general cooling, norepinephrine, ATP, adaptation
Received 15.04.2019.
For citation:
Patrakeeva V.P., Schtaborov V.A. Influence of background lympopolic on the reactions of systemic immunity during short-term total cooling. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2019, Vol. 16, no. 2, pp. 170–176. DOI: 10.22138/2500-0918-2019-16-2-170-176 (In Russ)
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Аuthors
Veronika P. Patrakeeva
Cand. Sci. (Biology), Leading Researcher, Laboratory of Environmental Immunology
patrakeewa.veronika@yandex.ru
Vyacheslav A. Shtaborov
Cand. Sci. (Biology), Senior Researcher, Laboratory of Environmental Immunology
schtaborov@mail.ru
Institute of Environmental Physiology of Federal Center for Integrated Arctic Research of the RAS acad. N.P. Laverova
Lomonosov ave. 249 Arkhangelsk Russian Federation 163061
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