Influence of Background Neutropeniа on Immune Reactions in Practically Healthy People with Short-Term General Cooling
DOI: 10.22138/2500-0918-2018-15-2-239-247
S.N. Balashovа, K.O. Pashinskaya
Institute of Environmental Physiology of Federal Center for Integrated Arctic Research Russian Academy of Sciences, Arkhangelsk, Russian Federation
Abstract. Aim. Тo determine the effect of neutropenia on the immune responses of practically healthy adults with short-term general cooling. Materials and methods. The study of peripheral venous blood parameters in 75 volunteers before and after short-term general cooling (5 minutes in a cold chamber at –25 ° C) was conducted. The concentrations of epinephrine and norepinephrine by test kits from IBL Hamburg (Germany), irisin – BioVendor (Czech Republic), endothelin-1 – Biomedica (Czech Republic), IL-17F and TNF-? – Bender MedSystems (Austria) were determined by the enzyme immunoassay on the Evolis automatic analyzer (Bio-RAD, France). Results and discussion. The content of neutrophilic granulocytes below 2^109 cells/l is associated with a decrease in the phagocytic activity of neutrophils and an increase in the blood levels of IL-17F and TNF-?. After a stay in the climatic chamber in the group of persons with neutropenia, there was a further decrease in the neutrophil count in the blood. The cells respond functionally to the cold factor, the functionally the most active and young, while the content of old neutrophils practically does not change. After a short-term cooling, regardless of the presence or absence of neutropenia, a decrease in the level of mature, activated, differentiated T cells and natural killers, as well as lymphocytes labeled for apoptosis, is recorded. A significant difference is revealed with respect to the level of decrease in activated T cells with the receptor for transferrin (CD71 +) in neutropenia. Conclusion. Reaction to cold exposure in the presence of neutropenia is characterized by inhibition of the reactions of catecholamines and endothelin-1, which are involved in ensuring adequate thermogenesis. In conditions of neutropenia, the level of reaction to cold of activated T-lymphocytes is inhibited. The effect of neutropenia on reactive changes in the content of irisin in the blood under conditions of short-term general cooling in the climatic chamber is not established.
Keywords: neutropenia, epinephrine, norepinephrine, cytokines, endothelin, irisin, total cooling
Received 09.04.2018
For citation:
Balashovа S.N., Pashinskaya K.O. Influence of Background Neutropeniа on Immune Reactions in Practically Healthy People with Short-Term General Cooling. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2018, Vol. 15, no. 2, pp. 239–247. DOI: 10.22138/2500-0918-2018-15-2-239-247 (In Russ)
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Authors
Svetlana N. Balashova
Cand. Sci. (Bio), Senior Researcher of the Laboratory of Regulatory Mechanisms of Immunity
ifpa-svetlana@mail.ru
Ksenia O. Pashinskaya
Post-graduate student of the Laboratory of regulatory mechanisms of immunity
nefksu@mail.ru
Institute of Environmental Physiology of Federal Center for Integrated Arctic Research Russian Academy of Sciences
Russian Federation, 163001, Arkhangelsk, Lomonosov ave., 249
