Effect of the cavitated chlorhexidine solution on the quantitative and species composition of the vaginal lactobacilli
DOI: 10.22138/2500-0918-2016-14-4-52-60
E.S. Voroshilina, E.E. Plotko, L.V. Hautin, D.L. Zornikov
Ural State Medical University, Yekaterinburg, Russian Federation;
Medical center «Garmonia», Yekaterinburg, Russian Federation
Abstract.
Object of the research was to evaluate the effect of the 0.05% cavitated chlorhexidine solution on the quantitative and species composition of vaginal lactobacilli.154 women in their reproductive age were included in the study. All women underwent vaginal treatment with 0.05% cavitated chlorhexidine solution using the AUZH-100 machine: time of the impact — 1–2 minutes, power — 6–8 units, amount of the solution used — 150–200 ml.State of the vaginal microbiocenosis and the amount of every species of lactobacilli were determined using PCR-RT. The study was conducted twice: before (during the phase of primary consults) and after treating vagina and exocervix with the 0.05%cavitated chlorhexidine solution.Depending on the prevailing lactobacilli species in their biocenosis the peatients were divided into five groups: with L. crispatus, L. iners, L. jensenii, L. gasseri, or L. vaginalis dominating. Retention or the increase in the cumulative amount of lactobacilli in microbiocenosis after treating vagina with the 0.05% cavitated chlorhexidine solution with L. crispatus or L. iners dominating was reported in 84% and 98% of cases respectively, and in 59% of cases with L. gasseri dominating.The amount and the list of the simultaneously detected species after the treatment did not change in any of the patients. In most of the women the prevailing lactobacillus species remained the same (77.7%), and the change in the dominating species was reported in 22.3% of the cases. In all the cases the dominating species changed to the species present in the microbiocenosis before, but in smaller amount, and the species prevailing before retained, but as the accompanying species. The change of the dominating species was more often reported if L. gasseri was dominating before (in 3 out of 6 patients).The effect of the 0.05% cavitated chlorhexidine solution on the composition of vaginal lactobacilli is limited and is determined by the original species composition of vaginal lactobacilli.
Keywords:
vaginal microbiocenosis, lactobacilli, cavitated chlorhexidine solution, real-time PCR, L. crispatus, L. iners, L. gasseri
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Authors
Voroshilina Ekaterina S.
Ural State Medical University
MD, docent
Russian Federation, Yekaterinburg, Klyuchevskaya str.17
voroshilina@gmail.com.
Plotko Evgeny E.
MFC Garmonia, Yekaterinburg
MD, Chief doctor
Russian Federation, 620026 Yekaterinburg, Tveritina str.16
plotko@garmonia-mc.ru
Hayutin Leonid V.
MFC Garmonia, Yekaterinburg
obstetrician-gynaecologist
Russian Federation, 620026 Yekaterinburg, Tveritina str.16
office@garmonia-mc.ru
Zornikov Danila L.
Ural State Medical University
Postgraduate student. Department of microbiology, virology and immunology.
Russian Federation, Yekaterinburg, Klyuchevskaya str.17
phenix520@yandex.ru
Received 05.09.2016
For citation:
Voroshilina E.S., Plotko E.E., Hautin L.V., Zornikov D.L. Effect of the cavitated chlorhexidine solution on the quantitative and species composition of the vaginal lactobacilli [Vlijanie kavitirovannogo nizkochastotnym ul'trazvukom rastvora hlorgeksidina na kolichestvennyj i vidovoj sostav laktoflory vlagalishha] Vestn. Ural. Med. Akad. Nauki. – Journal of Ural Medical Academic Science. 2016, no. 4, pp. 52–60. DOI: 10.22138/2500-0918-2016-14-4-52-60 [In Russ.]
