EFFECT OF VITAMIN-ANTIOXIDANT COMPLEXES ON DIFFERENT COMPONENTS OF THE HEMOSTATIC SYSTEM
DOI: 10.22138/2500-0918-2018-15-6-808-813
A.V. Samoylov, V.G. Solovyev, R.R. Galimov,
S.P. Kalashnikova, L.G. Nikonova
Khanty-Mansiysk State Medical Academy, Khanty-Mansiysk, Russian Federation
Abstract. Introduction. Excessive activation of continuous intravascular coagulation (CIC) and impossibility to correct this condition may lead to disseminated intravascular coagulation (DIC). Vitamin-antioxidant complexes are recommended among other nonspecific drugs to reduce the effect of hemocoagulation stress. Objective. To study the effect of a vitamin complex (VC) on hemostasis under the conditions of its activation. Materials and methods. The study was performed using random-bred albino rats. The number of animals in each comparison group was 12. Hemostasis was activated by cold stress. In order to correct the stress effect, one group of animals received VC during 21 days. To assess the platelet component of the hemostatic system, the platelet count was calculated and the coagulant activity released by the platelets was determined. The plasma component of hemostasis was evaluated by the following tests: activated partial thromboplastin time (aPTT), prothrombin time (PT), thrombin time (TT), antithrombin III activity (AT-III), content of soluble fibrin monomer complexes (SFMC) and plasma fibrinogen concentration. Results. After addition of a vitamin complex, platelete consumption decreased to zero, and total platelet coagulant activity increased. Changes in hypercoagulable states caused by stress effects were less pronounced, the consumption of AT-III was not observed. Conclusion. Additional administration of VC for three weeks decreased the effect of endogenous thrombinemia, which was triggered by a single cold exposure.
Keywords: stress, thrombinemia, acceleration of continuous intravascular coagulation, vitamins, platelets, plasmocoagulation
There is no conflict of interest.
Contact details of the corresponding author:
Anton V. Samoylov
acid2001@rambler.ru
Received 09.10.2018
For citation:
Samoylov A.V., Solovyev V.G., Galimov R.R., Kalashnikova S.P., Nikonova L.G. Effect of vitamin-antioxidant complexes on different components of the hemostatic system. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2018, Vol. 15, no. 6, pp. 808–813. DOI: 10.22138/2500-0918-2018-15-6-808-813 (In Russ).
REFERENCES
1. Bysheskiy, A. Sh. Vitamins and hemocoagulation. Sverdlovsk: Sredne-Uralskoe knizhnoe izdatelstvo, 1978. p. 126 (In Russ).
2. Plotnikov M.B., Ivanov I.S., Sidekhmenova A.V. The influence of dihydroquercetin and lipoic acid composition on the hemostasis in conditions of a model of lower limb chronic venous insufficiency in rats. Sibirskiy medicinskiy zhurnal. 2012. Vol. 27. No. 1. pp. 122-124. (In Russ).
3. Plotnikov M.B., Plotnikov D.M., et.al. Clinical efficacy of the new hemorheological drug ‘ascovertin’ in patients with dyscirculatory encephalopathy. Zhurnal nevrologii i psihiatrii. 2004. No. 12. pp. 33-37. (In Russ).
4. Plotnikov M.B., Pavlyukova E.N., et.al. Hemorheological and cardioprotective effects of ascovertin in patients with ischaemic heart disease. Tromboz, gemostaz i reologiya. 2005. No. 2. pp. 34-41. (In Russ).
5. Ralchenko, I.V. Lipid peroxidation and hemostasis. Ralchenko I.V., Rudzevich E.L., Rudzevich A.Yu., et.al. Hemostasis and lipid peroxidation in some experimental and clinical situations. Tyumen 2012. pp. 138-144. (In Russ).
6. Gerald F. Combs, Jr., James P. McClung. The Vitamins: Fundamental Aspects in Nutrition and Health. Academic Press., 2016., P. 628.
7. Byshevskiy A.Sh., Tersenov O.A. Biochemistry for doctors. — Ekaterinburg: Uralskiy rabochiy, 1994. pp. 136-138. (In Russ).
8. Shapovalova E.M., Vasilev L.A., Kondakov V.V., et.al. Vitamins A, E, C, B-5 and B-12, hemostasis and lipid peroxidation. Impact of the most important vitamin-antioxidant complexes on continous intravascular coagulation and thrombin tolerance — M: Medicinskaya kniga. 2009. pp. 12-47. (In Russ).
9. Xiaole Su, MD, Lijun Liu, MD. In reply to ‘Vitamins E and C may differ in their effect on contrast-induced acute kidney injury’. Am. J. Kidney Dis., 2017., Vol. 69(5), P 705-711.
10. Vasiliev L.A. Vitamins A, E, C, B-5 and B-12, hemostasis and lipid peroxidation. Vasilev L.A., Galushko M.G., Shchepeteva O.K., et.al. Impact of the most important vitamin-antioxidant complexes on continous intravascular coagulation and thrombin tolerance. — M: Medicinskaya kniga, 2009. pp. 8-12. (In Russ).
11. Byshevskiy A.Sh. Vitamin B12 and hemostasis. Byshevskiy A.Sh., Volosatov A.A., Karpova I.A., et.al. Fundamental Research. 2013. No. 2-1. pp. 221-¬226. (In Russ).
12. Jerneren F., Elshorbagy A.K., Oulhaj A., et al. Brain atrophy in cognitively impaired elderly: the importance of long-chain ω-3 fatty acids and B vitamin status in a randomized controlled trial. Am. J. Clin. Nutr. 2015., Vol. 102 (1). pp. 215-221.
13. Kim S.J., Zuchniak A., Sohn K.J., et. al. Plasma folate, vitamin B-6, and vitamin B-12 and breast cancer risk in BRCA1- and BRCA2-mutation carriers: a prospective study. Am. J. Clin. Nutr. 2016. Vol. 104 (3). pp. 671¬677.
14. Ulvik A., Pedersen E.R., Svingen GF, et al., Vitamin B-6 catabolism and long-term mortality risk in patients with coronary artery disease. Am. J. Clin. Nutr. 2016. Vol. 103 (6). pp. 1417-1425.
15. Gylling B., Myte R., Schneede J., et. al. Vitamin B-6 and colorectal cancer risk: a prospective population-based study using 3 distinct plasma markers of vitamin B-6 status. Am. J. Clin. Nutr. 2017. Vol. 105 (4). pp. 897-904.
16. Andrianova M.Yu., Roytman E.V., Isaeva A.M., et.al. Prevention of hyperhomocysteinemia: pathogenetic and clinical considerations. Arkhiv vnutrenney meditsiny. 2014. No. 4(18). pp. 32-38. (In Russ).
17. Kruglova O.G., Dorovskih V.A., Tihanov V.I., et.al. Comparative characteristic of dihydroquercetin and vitamin E effect on lipid peroxidation products in the blood of laboratory animals after cold exposure. Bulletin. 2011. No. 40. pp. 71-73. (In Russ).
18. Pan P.H., Lin S.Y., Wang Y.Y, et al. Protective effects of rutin on liver injury induced by biliary obstruction in rats. Free Radical Biology & Medicine. 2014. Vol. 73. pp. 106-116.
19. Yang J., Guo J., Yuan J. In vitro antioxidant properties of rutin. LWT — Food Science and Technology. 2008. Vol. 41. pp. 1060-1066.
20. Balakina A. S., Trusov N. V., Avreneva L. I., et.al. Effect of rutin and hesperidin on the expression of Nrf2 gene and the activity of heme oxygenase-1 and NAD(P)H-quinone oxidoreductase at their separate and combined action. Voprosy pitaniya. 2016. No. 3. pp. 18-27. (In Russ).
Authors:
Anton V. Samoylov
Postgraduate Student
Department of Medical and Biological Chemistry
acid2001@rambler.ru
Vladimir G. Solovyev
Dr. Sci. (Med.), Professor, Head of the Department of Medical and Biological Chemistry
vg_solovev@mail.ru
Rustam R. Galimov
Postgraduate Student
Department of Medical and Biological Chemistry
grr-86@mail.ru
Svetlana P. Kalashnikova
Cand. Sci. (Biological), Senior Lecturer, Professor of the Department of Medical and Biological Chemistry
kalashnikovas81@mail.ru
Lubov G. Nikonova
Cand. Sci. (Pharmaceutical), Senior Lecturer, Professor of the Department of Medical and Biological Chemistry
nikonovalg-hm@mail.ru
Khanty-Mansiysk State Medical Academy
Mira St. 40, Khanty-Mansiysk, Russian Federation, 628011
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)