Influence of Short-Term General Cooling on the Functional State of Immunokompetent Cells of Peripheral Blood f the Human

V.P. Patrakeeva, A.V. Samodova

DOI: 10.22138/2500-0918-2017-14-4-362-368

Institute of Environmental Physiology of Federal Center for Integrated Arctic Research Russian Academy of Sciences, Arkhangelsk, Russian Federation

Abstract. Aim. Determination of the effect of short-term general cooling on the content of immunocompetent cells of human peripheral blood. Materials and methods. A study was made of the content of immunocompetent cells of peripheral blood in 39 volunteers before and after short-term general cooling (for 5 minutes in a cold chamber at –25 °C). The concentration of epinephrine, norepinephrine by test kits from IBL Hamburg (Germany) and the level of irisin — BioVendor (Czech Republic) were determined by the enzyme immunoassay on the automatic enzyme immunoassay analyzer Evolis (Bio-RAD, France). Results. The conducted researches have shown that the reaction from the immune system can be manifested by multidirectional changes in the level of lymphocytes of peripheral blood. Thus, in 35.01±1.47% of the people surveyed, the decrease in lymphocyte count was more than 1.5 times, in 57.5±1.89% of cases — there were no changes in the concentration of these cells. It is established that one of the factors that ensure the characteristics of the response to cold is the initial background level of the immune system and catecholamines. Conclusion. Thus, in 57.5% of the surveyed patients, with a general short-term cooling, the stability of hemodynamics, the concentration of circulating neutrophils, monocytes, lymphocytes, the levels of activated immunocompetent cells is preserved against the background of the absence of changes in the content of catecholamines. 35% of the people surveyed even react to this short-term cooling with the formation of an adaptive reaction of redistribution of blood cells from circulating to marginal, activation of migration and recycling of immunocompetent cells with an increase in the synthesis of ATP in lymphocytes. Migration of leukocytes of lymphocytes occurs due to activated cells; migrate predominantly activated T-lymphocytes of the phenotypes CD25 +, CD71 +, HLADRII + and CD10 +, which are subsequently capable of blast transformation. Recirculate small lymphocytes, which are predominantly T-cells of the reserve pool, capable in the future of both blast transformation and differentiation.

Keywords: cold, adaptation, lymphocytes, adrenaline, norepinephrine, irisin, individual sensitivity

Received 24.11.2017

For citation:
Patrakeeva V.P., Samodova A.V. Influence of short-term general cooling on the functional state of immunokompetent cells of peripheral blood of the human. Vestn. Ural. Med. Akad. Nauki = Journal of Ural Medical Academic Science. 2017, Vol. 14, no. 4, pp. 362–368. DOI: 10.22138/2500-0918-2017-14-4-362-368 (In Russ)

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Authors
Veronika P. Patrakeeva
Сand. Sci. (Bio), Head of the Laboratory Ecological immunology
patrakeewa.veronika@yandex.ru

Anna V. Samodova
Сand. Sci. (Bio), Head of the Llaboratory Regulatory mechanisms of immunity
annapoletaeva2008@yandex.ru

Institute of Environmental Physiology of Federal Center for Integrated Arctic Research Russian Academy of Sciences
Russian Federation, 163061, Arkhangelsk, Lomonosov ave., 249