INFLUENCE OF PSG PEPTIDES ON THE MORPHOFUNCTIONAL STATE OF THE SPLEEN IN ALLOGENOUS BONE MARROW CELL TRANSPLANTATION

Yu.A. Charushina¹, N.P. Loginova¹, N.I. Gulyaeva¹, V.S. Prokudin², A.V. Lyubimov⁴, S.A. Zamorina³

DOI: 10.22138/2500-0918-2022-19-3-222-230

¹ Perm State Medical University named after academician E.A. Wagner
Ministry of Health of the Russian Federation, Perm, Russian Federation;
² State public health institution of special kind «Perm Regional Bureau of Forensic Medical Examination», Perm, Russian Federation;
³ Perm State National Research University, Perm, Russian Federation;
⁴ University of Illinois, Chicago, USA

Abstract. It is known that proteins associated with pregnancy provide immune tolerance during pregnancy. One of them is pregnancy-specific β1-glycoprotein (PSG), which has a pronounced immunomodulatory effect. Tetrapeptide fragments (YECE, YQCE, YVCS, and YACS) have been identified in the primary structure of PSG that have immunopharmacological potential. The aim of the study was to evaluate the effect of short peptide fragments of PSG on the morphological and immunohistochemical state of the spleen and its compartments during allogeneic transplantation of a suspension of red bone marrow cells in an in vivo experiment in Wistar rats. It was found that intraperitoneal injection of bone marrow cells in the dynamics of the experiment (35 days) caused splenic hyperplasia with accumulation of lymphoid cells and macrophages in the functional areas of the organ. Administration of short peptide fragments of PSG against the background of allogeneic bone marrow transplantation promotes activation of cells of the immune system in the spleen toward their proliferation (Ki-67) and differentiation, while reducing the content of macrophages (CD68). Thus, short peptide fragments of PSG stabilize proliferative processes and promote the development of adaptive responses in response to an allogeneic graft.

Keywords: allogeneic transplantation, red bone marrow, pregnancy proteins, spleen, macrophages, CD68, proliferation, Ki-67

There is no conflict of interest.
Contact details of the corresponding author:
Yulia A. Charushina
julia_pochta@mail.ru
Received 24.07.22
For citation:
Charushina Yu.A., Loginova N.P., Gulyaeva N.I., Prokudin V.S., Lyubimov A.V., Zamorina S.A. Influence of PSG peptides on the morphofunctional state of the spleen in allogenous bone marrow cell transplantation. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2022, Vol. 19, no. 3, pp. 222–230. DOI: 10.22138/2500-0918-2022-19-3-222-230 (In Russ)

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Authors
Yulia A. Charushina
Perm State Medical University named after I.I. academician. E.A. Vagner
Lecturer at the Department of Histology, Embryology and Cytology
29 st. Petropavlovskaya Perm Russian Federation
julia_pochta@mail.ru

Natalya P. Loginova
Perm State Medical University named after I.I. academician. E.A. Vagner
Doctor of Medical Sciences, Associate Professor, Head of the Department of Histology, Embryology and Cytology
29 st. Petropavlovskaya Perm Russian Federation
natalitsa@yandex.ru

Natalia I. Gulyaeva
Perm State Medical University named after I.I. academician. E.A. Vagner
Candidate of Medical Sciences, Associate Professor, Leading Researcher, Central Scientific Research Laboratory
29 st. Petropavlovskaya Perm Russian Federation
bizon55@mail.ru

vprokudin72@mail.ru

Alexander V. Lyubimov
University of Illinois, Chicago, USA
Scientific Supervisor of the Laboratory of Toxicological Research of the Department of
Pharmacology, Professor, Doctor of Medicine
808 South Wood St., room 1306, Chicago, IL 60612], 1-312-523-3894
alyubimov1@gmail.com

Svetlana A. Zamorina
Perm State National Research University
Doctor of Biological Sciences, Leading Researcher Laboratory of Ecological Immunology, Professor, Department of Microbiology and Immunology, Faculty of Biology
13 st. GolevaP\ Perm Russian Federation 614081
mantissa7@mail.ru