The Effect of Different Doses of Gemopoetic Stem Cells when Conducting Co-Transplantation of MSC on the Regeneration of Myeloid Tissue after Exposure to Ionizing Radiation
Grebnev D. U., Jastrebov A. P., Maclakova I. U.
State Budget Educational Establishment of the highest Professional Education ”The Ural State Medical Academy” of the Ministry of Health Care, Yekaterinburg, Russian Federation;
State Budget Establishment of the Health Care Department of Sverdlovsk Region “The Institute of the medical cell technologies”, Yekaterinburg, Russian Federation
Abstract.
Experimental study on the influence of various doses of gemopoetic stem cells when conducting co-transplantation with multipotent mesenchymal stromal cells on the regeneration of myeloid tissue after exposure to ionizing radiation. Research has revealed not only dose-dependent effect of transplanted HSCs, but also to identify the minimum effective dose of 330 thousand cells/kg, which would allow at joint application with multypotent mesenchimal stromal cells activate tissue regeneration.
Keywords:
multypotent mesenchimal stromal cells, gemopoetic stem cells, regeneration, myeloid tissue
REFERENCES
1. Beljaev N.N., Rysuly M.R., Isabekova A.S. Influence of IL-3 on Regenerative Inductive Activity of Haematopoietic Stem Cells of Marrow at the Experimental Myocardial Infarction. Kletochnaja Transplantologija i Tkanevaja Inzhenerija — Cellular Transplantology and Tissue Engineering. 2009. Vol. 4. No. 4. pp. 50-55.
2. Grebnev D. Yu., Мaklakova I. Yu., Yastrebov A. P. The opportunity to use combined stem cells transplantation for haemopoesis activation in the old and mature laboratory animals under the conditions of ionizing radiation. Uspehi Gerontologii — Advances in Gerontology. 2014. Vol. 27, No. 2. pp. 348-352.
3. Isaev A.A., Melihova B.C. Application of cages of an umbilical blood in clinical practice. Kletochnaja Transplantologija i Tkanevaja Inzhenerija — Cellular Transplantology and Tissue Engineering. 2008. Vol. 3, No. 1. pp. 34-41.
4. Sazonov S.V. Osobennosti sostojanija proliferativnyh processov v uslovijah vozrastnoj involjucii organizma. Ph.D. [Features of a Condition of Proliferative Processes in the Conditions of an Age Involution of an Organism. Ph.D. Yekaterinburg. 1999. 372 p.
5. Ufimtceva A.I., Kanov E.V. Characterization and Ex Vivo Expansion Umbilical Cord Blood Hematopoietic Stem and Progenitor Cells. Kletochnaja Transplantologija i Tkanevaja Inzhenerija — Cellular Transplantology and Tissue Engineering. 2012. Vol. 7, No. 4. pp. 21–27.
6. Aparicio-Vergara, M. Bone marrow transplantation in mice as a tool for studying the role of hematopoietic cells in metabolic and cardiovascular diseases. M. Aparicio-Vergara, R. Shiri-Sverdlov, G.de Haan. Atherosclerosis. 2010. Vol. 213. p. 335–44.
7. Caplice, N. M. The future of cell therapy for acute myocardial infarction. N. M.Caplice. Nature Clinical Practice Cardiovascular Medicine. – 2006. – Vol. 3. – P.129–132.
8. Gluckman E., Rocha V. Cord blood transplantation: state of the art. Haematologica 2009; 94 (4); 451-4.
Authors
Grebnev Dmitrij U.
dr-grebnev77@mail.ru
Maclakova Irina U.
makliu@mail.ru
Yastrebov Anatoly P.
dr-grebnev77@mail.ru
State Budget Educational Establishment of the highest Professional Education ”The Ural State Medical Academy” of the Ministry of Health Care, Yekaterinburg, Russian Federation;
State Budget Establishment of the Health Care Department of Sverdlovsk Region “The Institute of the medical cell technologies”, Yekaterinburg, Russian Federation
Russian Federation, 620000, Yekaterinburg, st. Repin, 3