EFFECT OF COMBINED TRANSPLANTATION OF MULTIPOTENT MESENCHYMAL STROMAL AND HEMATOPOIETIC STEM CELLS ON LIVER REGENERATION AFTER LIVER RESECTION IN OLD LABORATORY ANIMALS

DOI: 10.22138/2500-0918-2020-17-2-139-148

I.Yu. Maklakova, V.V. Bazarniy, D.Yu. Grebnev

Ural State Medical University, Yekaterinburg, Russian Federation;
Institute of Medical Cell Technologies, Yekaterinburg, Russian Federation

Abstract. The aim of this study was to study the changes in the morphological and functional state of the liver of old laboratory animals after its resection against the background of combined transplantation of multipotentmesenchymal stromal and hematopoietic stem cells.Materials and methods. The studies were performed on old laboratory animals, male mice of the age of 16-17 months. Resection of 2/3 of the liver was performed in old laboratory mice according to the method of C. Mitchell and H. Willenbring. One hour after subtotal resection, a combined transplantation of two types of cells was performed: multipotentmesenchymal stromal cells (at a dose of 4 million cells / kg) and hematopoietic stem cells (at a dose of 330 thousand cells / kg). The experiments on obtaining the MMSC and HSC culture were performed from the placenta chorion of 10 laboratory female mice 3-4 months old, weighing 20-25 g, gestational age 18 days. Assessment of liver regeneration was carried out on days 1, 3, 7 after resection of the liver by analyzing morphometric parameters of the liver and biochemical parameters of peripheral blood.Results. Studies have shown the activation of protein synthesizing liver function in old animals, a decrease in the level of cytolysis enzymes, an increase in the activity of cellular regeneration (increase in the hepatocyte nucleus area, the number of binuclear cells) of liver regeneration after resection against the introduction of stem cells.

Keywords: liver resection, multipotentmesenchymal stromal cells, hematopoietic stem cells, liver regeneration, aging

Received 10.01.2020

For citation:
Maklakova I.Yu., Bazarniy V.V., Grebnev D.Yu. Effect of combined transplantation of multipotent mesenchymal stromal and hematopoietic stem cells on liver regeneration after liver resection in old laboratory animals. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 2, pp. 139–148. DOI: 10.22138/2500-0918-2020-17-2-139-148 (In Russ)

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Authors
Irina Y. Maklakova
Ural State Medical University
Cand. Sci. (Med.), Associate Professor of the Department of pathological physiology,
Senior researcher at the Institute of Medical Cell Technologies
3 Repin str. Yekaterinburg, Russian Federation, 620028
makliu@mail.ru

Vladimir V. Bazarnyi
Ural State Medical University
Dr. Sci. (Med.), Professorof the Department of clinical laboratory diagnostics and bacteriology
3 Repin str. Yekaterinburg, Russian Federation, 620028
vlad-bazarny@yandex.ru

Dmitry Y. Grebnev
Ural State Medical University
Dr. Sci. (Med.), Head of the Department of pathological physiology, Senior researcher at the Institute of Medical Cell Technologies
3 Repin str. Yekaterinburg, Russian Federation, 620028
dr-grebnev77@mail.ru

 
 
 

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