Influence of carbon nanotubes on the structure of myelin nervous fibres at the resection of the sciatic nerve
Korotkich A.G., Sazonov S.V., Tuponogov S.N., Leontiev S.L.
Abstract. There are no data on efficiency of use of carbon nanotubes for regeneration of nervous fibers in experiment on laboratory animals in literature. The work purpose — to study influence of one-wall carbon nanotubes on process of regeneration of myelin nervous fibers of peripheral nerves in the experimental in vivo model. The injury of a nerve was carried out in model on laboratory rabbits. In total 29 animals who have been distributed on two selections are operated. On the excised sciatic nerve imposed the conduit from a teflon vascular artificial limb which was filled with one-wall carbon nanotubes. The collateral (right) extremity served control on which the same surgery, as on skilled only without application of carbon nanotubes was carried out. Animals from group No. 1 in three months were brought out of experiment, out of group No. 2 in 6 months. Made semi-thin histologic cuts which painted hematoxylin-eozinom and investigated at light microscopy. For research took three sites: proximal, distalny and a site in the field of a vascular artificial limb. Measured diameter of myelin nervous fibers of skilled and control extremities of group No. 1 in a disteel and proximal site of a nerve on two axes. It was succeeded to establish that at application of carbon nanotubes diameter of myelin nervous fibers in disteel sites of a skilled extremity more in comparison with control is reliable.
Keywords: regeneration, peripheral nerve, one-wall carbon nanotubes, diameter of myelin nervous fibers
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Autors
Korotkich Anna G.
Ural state medical university
department of histology, cytology and embryology, assistant to department
Russian Federation, Yekaterinburg, Onufriyev Str. 20a, 620149
korotkich.hist@yandex.ru, ph. 8 (343) 2148792
Sazonov Sergey Vladimirovich
Ural state medical university
department of histology, cytology and embryology, head of the department, MD, professor
Russian Federation, Yekaterinburg, Onufriyev Str. 20a, 620149
prof-s,sazonov@yandex.ru
Tuponogov Sergey N.
GBUZ ODKB№1 of Sverdlovsk region,
children's surgeon-oncologist, doctor of higher category,
Russian Federation, Yekaterinburg, Serafima Deryabina Str. 32
Leontyev Sergey L.
GAUZ SO Institute of Medical Cell Technologies
Russian Federation, Yekaterinburg, 620026, K. Marksa str., 22a
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