THE EFFECT OF VITAMIN D3 IN THE COMPOSITION OF RECTAL SUPPOSITORIES OF THE ORIGINAL COMPOSITION ON THE INDICATORS OF FREE RADICAL OXIDATION IN THE MUCOUS MEMBRANE OF THE COLON IN THE DYNAMICS OF EXPERIMENTAL ULCERATIVE COLITIS
DOI: 10.22138/2500-0918-2020-17-1-42-52
M.V. Osikov, E.V. Simonyan, M.S. Boyko
Federal State Budgetary Educational Institution of Higher Education «South Ural State Medical University» of the Ministry of Health of the Russian Federation, Chelyabinsk, Russian Federation
Abstract. Free radical oxidation plays an important role in the pathogenesis of ulcerative colitis (UC). Active oxygen species (ROS) trigger lipid peroxidation (LPO) with the formation of oxidative stress products. Basic therapy of UC is accompanied by side effects and is not effective in 30% of patients, in connection with which it is necessary to search for new therapeutic approaches. Vitamin D3 has antioxidant, anti-inflammatory and immunomodulating properties, is effective in some autoimmune diseases, which is a prerequisite for its use in UC. The aim of the work was to study the effect of vitamin D3 in the composition of rectal suppositories of the original composition on the content of LPO products in the mucous membrane of the large intestine in experimental UC. Materials and methods. Work was performed on 49 Wistar rats; UC was simulated by two-stage administration of 3% oxazolone; vitamin D3 was used every 12 hours in the composition of rectal suppositories based on polyethylene glycol. Clinical status was assessed using the Disease activity index (DAI) scale, and the content of lipid peroxidation products was determined by the extraction-spectrophotometric method. Results. It was established that with UC on day 2, 4, and 6, DAI rises, which is associated with the content of primary, secondary, and final LPO products in the heptane and isopropanol phases of the lipid extract of the colon mucous membrane homogenate. A new dosage form was developed and a complex of pharmaceutical and technological studies of rectal suppositories of the original composition containing 10% aqueous solution of vitamin D3 was carried out. Their use in experimental UC on the 4th and 6th day of observation leads to a decrease in DAI and a partial restoration of the content of primary, secondary, and final LPO products in the heptane and isopropanol phases of the lipid extract of the colon mucous membrane homogenate.
Keywords: ulcerative colitis, free radical oxidation, lipid peroxidation, vitamin D3, rectal suppositories
Received 10.12.2020г
For citation:Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 1, pp. 42–52. DOI: 10.22138/2500-0918-2020-17-1-42-52 (In Russ)
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Authors
Mikhail V. Osikov
Dr. Sci. (Med.), Professor, Head of Department of Pathological physiology
prof.osikov@yandex.ru
Elena V. Simonyan
Cand. Sci. (Pharmaceutical), Associate Professor, Head of the Department of Pharmacy
and Chemistry
elenasimonian@yandex.ru
Margarita S. Boyko
Assistant of the Department of Pathological Physiology
ri-tochka9@list.ru
Federal State Budgetary Educational Institution of Higher Education «South-Ural State
Medical University» of the Ministry of Healthcare of the Russian Federation
64 Vorovsky ul. Chelyabinsk Russian Federation 454092