AGE FEATURES OF MORPHOGENETIC PROPERTIES OF LYMPHOID CELLS OF THE SPLEEN
DOI: 10.22138/2500-0918-2020-17-2-149-160
S.V. Sazonov
Institute of Medical Cell Technologies, Yekaterinburg, Russian Federation;
Ural State Medical University, Yekaterinburg, Russian Federation
Abstract. Aim. The aim of the study is to determine the features of the morphogenetic properties of the spleen lymphoid cells in animals of different age groups. Materials and research methods. In the experiments, a model of adoptive transfer of lymphoid cells of the spleen of nephrectomized animals from different age groups was used. The studies were carried out on male Wistar rats of mature (8-10 months, weighing 200-250 g) and old (19-22 months, weighing 400-500 g) age. Study groups: control group — model of adoptive transfer of splenocytes from mature animals to mature ones; second — experimental group: model of adaptive transfer of lymphoid cells from old — old animals; the third group — transplantation of lymphoid cells from old animals — to mature ones; the fourth group — transplantation of lymphoid cells from mature animals — to old ones. The morphogenetic properties of the spleen cells of nephrectomized animals from different age groups were studied 19 hours after unilateral nephrectomy (donor interval). The resulting suspension was injected intravenously into recipients at 400×10³ cells per 0.2 kg of animal weight. 8 hours before slaughter, the animals received a single intraperitoneal dose of 2 mg/kg of vinblastine. Recipients were sacrificed 40, 48 and 56 hours after splenocyte transfer. Research results. Transplantation of splenocytes obtained from old donors to old recipients did not reveal any stimulation of proliferative processes in the epithelium of the kidney tubules of the latter. The transfer of the same splenocytes to mature donors is accompanied by the manifestation of their morphogenetic function. At the same time, the activity of proliferative processes in the kidneys of the recipients was not less pronounced than in the control group.
Keywords: splenocytes, adoptive transfer, morphogenetic properties, age characteristics, nephrectomy, immunohistochemical study, statmokinetic index
Received 29.09.2020
For citation:
Sazonov S.V. Age features of morphogenetic properties of lymphoid cells of the spleen. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2020, Vol. 17, no. 2, pp. 149–160. DOI: 10.22138/2500-0918-2020-17-2-149-160 (In Russ)
REFERENCES
1. Yastrebov A.P. Some results and prospects of studying the mechanisms of tissue regeneration when exposed to the body of extreme factors. Essays on experimental pathophysiology. Yekaterinburg: Publishing house «SV-96». 1999.S. 13-26. (In Russ.)
2. Babaeva A.G. Immunology of processes of adaptive growth, proliferation and their disorders / A.G. Babaeva, E.A. Zotikova. Moscow: Nauka, 1987.207 p. (In Russ.)
3. Babaeva A.G. The unity and opposition of the cytogenetic activity of lymphocytes and their antibody-forming function during the restoration processes in organs. Bulletin of Experimental Biology and Medicine. 1999, 128 (11), pp. 484 — 490. (In Russ.)
4. Sazonov S.V. T-lymphocytes are regulators of cell proliferation activity in tissue. Bulletin of the Ural Medical Academic Science. Ekaterinburg, 2007, 1 (15), pp. 14-20. (In Russ.)
5. Sazonov S.V., Yastrebov A.P. Possibilities of lymphocyte transplantation to restore regeneration in organs during aging of the body. Hospital messenger. Ekaterinburg, 2006, 4, pp. 24-29. (In Russ.)
6. Yastrebov A.P., Grebnev D.Yu., Maklakova I.Yu., Osipenko A.V., Makeev O.G., Meshchaninov V.N., Sazonov S.V., Vechkaeva I.V., Syrnev V.A. On the participation of stem cells in the regulation of regenerative processes in extreme injuries. Collection of scientific works «Cell technologies for practical health care, 2015, pp. 108-113. (In Russ.)
7. Blyakher M.S., Gutorova N.M., Fedorova I.M. and others. The number of subpopulations of lymphocytes in the spleen and the level of proliferation of hematopoietic tissue in mice during surgical interventions. Microbiology and Immunology, 1996, 3, p. 301 — 303. (In Russ.)
8. Sazonov S.V. Age features of the state of regenerative processes in tissues. Essays on experimental pathophysiology. Yekaterinburg: Publishing house «SV-96». 1999.S. 352-365. (In Russ.)
9. Sazonov S.V. Analysis of proliferative processes in organs with different mechanisms of cellular renewal during aging. Morphology, 2016, 149 (3), P. 177. (In Russ.)
10. Sazonov S.V. The state of the mitotic cycle in the cells of the renal epithelium after unilateral nephrectomy. Collection of scientific papers of the YIII All-Russian conference on cell pathology, November 11-12, 2010, Moscow, pp. 210-212. (In Russ.)
11. Sazonov S.V. Features of proliferative processes in tissues with a high rate of cellular renewal during age-related involution of the organism. Morphology, 2012, Vol. 141, No. 3, P. 136. (In Russ.)
12. Sazonov S.V., Leontiev S.L., Yastrebov A.P. Age features of the temporal parameters of the cell cycle in the epithelial cells of parenchymal organs in rats. Collection of scientific papers of a scientific conference with international participation: «Topical issues of morphogenesis in health and disease», Moscow, April 6-7, 2016, pp. 157-158. (In Russ.)
13. Babaeva A.G. Recent trends in regeneration research. Life Sci. N.Y. Lond. 1989. Vol. 172. pp. 121-128.
14. Sazonov S.V., Popov A.M., Lisikh Yu.I., Popugailo M.V. Influence of lymphocytes on the proliferation of renal epithelium in hypothermia. Hospital Bulletin, Yekaterinburg, 2006, No. 4, pp. 32-38. (In Russ.)
15. Sazonov S.V., Shamshurina E.O., Beresneva O.Yu., Kurumchina S.G., Valamina I.E. Changes in morphogenetic properties of transplanted T-lymphocytes during aging. Materials of the III All-Russian symposium with international participation «Actual issues of tissue and cell transplantology», Moscow, CITO, April 25-26, 2007 (In Russ.)
16. Sazonov S.V., Popov A.M., Lisikh Yu.I., Popugailo M.V. Morphogenetic activity of transplanted lymphoid cells in hypothermia. Materials of the III All-Russian symposium with international participation «Actual issues of tissue and cell transplantology», Moscow, CITO, April 25-26, 2007 (In Russ.)
17. Sazonov S.V. Morphogenetic properties of lymphoid cells during age-related involution of the organism. Allergology and Immunology, 2008, 3 (9), P. 123. (In Russ.)
18. Sazonov S.V., Yastrebov A.P., Leontiev S.L. The state of cell regeneration after unilateral nephrectomy under the action of cold on the body. Collection of scientific papers of the All-Russian Scientific Conference «Regenerative Biology and Medicine», Moscow, 2011, pp. 136-137. (In Russ.)
19. Grebnev D.Yu., Yastrebov A.P., Maklakova I.Yu., Sazonov S.V., Leontiev S.L. Comparative analysis of the regeneration of the jejunal epithelium of mature and old laboratory animals under conditions of acute blood loss on the background of stem cell transplantation. Cell transplantology and tissue engineering, 2012, T.YII, No. 2, pp. 22-23. (In Russ.)
20. Sazonov S.V., Yastrebov A.P., Leontiev S.L. Influence of transplanted T-lymphocytes on the state of regenerative processes in organs during aging of the body. Cell transplantology and tissue engineering, 2012, T.YII, No. 2, P. 46. (In Russ.)
21. Flaherty D., Wagner C., Gross C. Aging and lymphocyte subsets in the spleen and peripheral blood of the Sprague-Dawley rat. Immunopharmacol. 1997, Vol. 2, P. 185-195.
22. Haynes L., Linton P. J., Swain S. L. Age-related changes in CD4 T cell of T cell receptor transgenic mice. Mech. Ageing Dev., 1997, Vol.93 (1-3), P.95-105.
23. Proust J. J., Quadri R. A., Arbogast A., Phelouzat M. Mecanismes moleculaires du dysfonctionnement lymphocytaire lie a l'age, Pathol. Biol. Paris, 1996, Vol. 44 (8), P. 729-236.
24. Mora J.R., von Andrian U.H. T-cell homing specificity and plasticity: new concepts and future challenges. Trends Immunol. 2016. 27 (5), Р. 235-43.
25. Sazonov S.V., Konyshev K.V. The use of adoptive transfer of lymphoid cells to stimulate proliferative processes during age-related involution of the body. Cytology, 2011, T. 53, No. 9. S. 735-736. (In Russ.)
26. Sazonov S.V., Brilliant A.A., Konyshev K.V. Standardization of immunohistochemical determination of the expression level of Ki-67 in cells of various tissues. Morphology, 2017, Vol. 151, No. 3, P. 100. (In Russ.)
27. Sazonov S. V. Determination of the level of proliferation in the tissues of organs during the immunohistochemical study of Ki-67. Morphology, 2018, T.153, No. 3, P. 242. (In Russ.)
28. Sazonov S.V., Yastrebov A.P. The state of proliferation processes in the renal epithelium after unilateral nephrectomy. Morphology, 2010, Volume 137, No. 4, P. 167. (In Russ.)
Author
Sergey V. Sazonov
Ural State Medical University, Department of Histology, Cytology and Embryology
Doctor of Medical Sciences, Professor
Institute of Medical Cell Technologies
Deputy Chief Physician for Science
3, st. Repin, Yekaterinburg, Russian Federation, 620028
prof-ssazonov@yandex.ru