INTERRELATION OF THE FUNCTIONAL STATE OF ERYTHROCYTES AND LYMPHOCYTES OF PERIPHERAL BLOOD IN HEALTHY PEOPLE
DOI: 10.22138/2500-0918-2018-15-2-205-210
V.P. Patrakeewa
Institute of Environmental Physiology of Federal Center for Integrated Arctic Research Russian Academy of Sciences, Arkhangelsk, Russian Federation
Abstract. The aim of the study was to determine the relationship between the functional state of erythrocytes and lymphocytes in normal and with lymphopenia. Materials and methods. A survey of 77 people (18–22 years old), residents of the city of Arkhangelsk. In the peripheral blood, a hemogram was determined on the hematological analyzer XS-1000i (Sysmex, Japan), leukogram. The expression of markers CD3, CD4, CD8, CD16, CD23, CD10, CD71, CD25, HLADRII and CD95 was determined on lymphocytes. In the peripheral blood, the concentration of catecholamines, lactate, pyruvate, triglycerides, transferrin, iron level, sTfR was determined by unified methods and the results were evaluated using a spectrophotometer «Multiscan MS» (Finland) and StatFax analyzer (USA). Results. According to the results of the studies, two groups were formed in accordance with the level of absolute lymphocyte count: the first group with lymphopenia — 29.87±0.62% of all examined (23 people), the second group (control) 54 people. In the group with lymphopenia, lower levels of hematologic indices are recorded. Data on the metabolic supply of lymphocytes indicate a cellular energy deficit in the presence of signs of tissue hypoxia. The conclusion. Thus, the content of erythrocytes and the oxygen supply of tissues play a significant role in the functioning of lymphocytes, the regulation of their metabolic activity. Reduction of erythrocytes, when the concentration of sTfR increases in the peripheral blood with a parallel increase in pyruvate content, reflect the state of tissue hypoxia and energy deficit of cells. In this case, inhibition of lymphoproliferation, suppression of activation of T-lymphocytes, with an increase in IgG production is recorded. Under such conditions, an adequate immune response can not be formed and all conditions for autosensitisation are created.
Keywords: lymphopenia, erythrocytes, homeostasis, hypoxia, energy deficiency, autosensitization
Received 09.04.2018
For citation:
Patrakeewa V.P. Interrelation of the Functional State of Erythrocytes and Lymphocytes of Peripheral Blood in Healthy People. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2018, Vol. 15, no. 2, pp. 205–210. DOI: 10.22138/2500-0918-2018-15-2-205-210 (In Russ)
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Author
Veronika P. Patrakeewa
Institute of Environmental Physiology of Federal Center for Integrated Arctic Research Russian Academy of Sciences Russian Federation
Cand. Sci (Biol.), Researcher of the Laboratory Ecological Immunology
Russian Federation, 163061, Arkhangelsk, Lomonosov Ave., 249
patrakeewa.veronika@yandex.ru
