INTERRELATION OF POLYMORPHIC VARIANTS OF THE VDR GENE WITH THE LEVEL OF VITAMIN D AND CYTOKINES IN PATIENTS WITH TYPE 2 DIABETES MELLITUS WITH OBESITY
DOI: 10.22138/2500-0918-2023-20-4-202-210
L.A. Safiullina, M.A. Vulf, N.D. Gazatova, L.S. Litvinova
Center for Immunology and Cellular Biotechnologies, Baltic Federal University. I. Kant, Kaliningrad, Russian Federation
Abstract. Vitamin D deficiency is associated with obesity, due to its role in the regulation of adipose tissue cells. Insufficient levels of vitamin D can contribute to the deterioration of the functioning of the pancreas, and be the cause of the development of type 2 diabetes mellitus (DM 2). In addition, vitamin D has a known anti-inflammatory effect, which has a protective effect on the onset of metabolic syndrome. Vitamin D plays an important role in the processes of distribution and activity of adipose tissue, exerting its action through receptors (VDR). Their study can help in understanding the mechanisms of action of vitamin D in various diseases. The aim of our study was to evaluate the relationship of polymorphic variants of the VDR gene with the level of vitamin D and cytokines (IL-8; IL-10) in the blood of obese patients with and without type 2 DM. Materials and methods. The study involved 150 patients who were divided into three separate groups: apparently healthy donors, obese patients without type 2 diabetes, and patients with type 2 diabetes and obesity. The polymorphism of the VDR gene (TaqI) was studied by PCR using a set of reagents manufactured by OOO NPF Sintol. The study of the level of vitamin D in the blood serum was carried out by ELISA, the content of inflammatory mediators in the blood plasma was determined by flow fluorimetry using commercial test systems (Bio-Plex Pro™ Human Inflammation Panel 1, 37-Plex). Results. A study of obese patients showed that carriers of the homozygous CC variant of the VDR-TaqI gene had higher levels of vitamin D than other polymorphic variants. It has been established that vitamin D deficiency in obese patients is associated with an increased level of the IL-8 chemokine and a low concentration of the anti-inflammatory cytokine IL-10 in the blood.
Keywords: vitamin D deficiency; type 2 diabetes; obesity; diseases associated with metabolic disorders
There is no conflict of interest.
Contact details of the corresponding author:
Larisa S. Litvinova
larisalitvinova@yandex.ru
Received 17.07.2023
For citation:
Safiullina L.A., Vulf M.A., Gazatova N.D., Litvinova L.S. Interrelation of polymorphic variants of the VDR Gene with the level of vitamin d and cytokines in patients with type 2 diabetes mellitus with obesity [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2023, Vol. 20, no. 4, pp. 202–210. DOI: 10.22138/2500-0918-2023-20-4-202-210 (In Russ)
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Authors
Larisa S. Litvinova
Doctor of Medical Sciences, Head (Director) of the Center; LLitvinova@kantiana.ru
Natalya D. Gazatova
Candidate of Biological Sciences, Head of the Laboratory of Experimental Blood Preparations
NGazatova@kantiana.ru
Maria A. Vulf
Candidate of Biological Sciences, Senior Researcher
MaVasilenko@kantiana.ru
Linara A. Safiullina
graduate student
lasafiullina@stud.kantiana.ru
Immanuel Kant Baltic Federal University Center for Immunology and Cellular Biotechnologies, Ministry of Education and Science of Russia
Russian Federation, Kaliningrad