INTERRELATION OF THE ENDOTHELIN-1 CONTAINMENT IN BLOOD AND THE CHARACTER OF IMMUNE REACTION OF THE PERSON FOR SHORT-TERM GENERAL COOLING
DOI: 10.22138/2500-0918-2018-15-2-299-308
A.V. Samodova, L.K. Dobrodeeva
Institute of Physiology of Natural Adaptations of the Federal State Budgetary Institution of Science of the Federal Research Center for Complex Arctic Studies. acad. N.P. Laverova Russian Academy of Sciences, Arkhangelsk, Russian Federation
Abstract: Aim of the study was to establish the relationship between the endothelin-1 blood content and the nature of the immune response in humans to the effect of short-term cooling during the minimum duration of daylight hours. Material and methods. A complex of immunological examination of 73 people who were practically healthy at the time of examination was performed, before and after being in the climatic chamber for 5 minutes at a temperature of –25°C during the minimum duration of daylight hours (January, February). A study of the concentration in the blood of a short peptide of endothelin-1 and cytokines was carried out by ELISA, the content of lymphocyte phenotypes in an indirect immunoperoxidase reaction using monoclonal antibodies and by flow cytometry. The number and ratio of hemogram cells, neutrograms, monocytes, lymphocytograms were counted in blood smears stained according to the Romanovsky-Giemsa method. Results. For short-term total cooling at –25°C for 5 minutes, 20.54% of the subjects reacted by a decrease in the total content of lymphocytes and their activated forms. It was found that the lymphocyte content and the level of their decrease depend on the initial concentration of endothelin-1: in persons with a high baseline level of the peptide, the lymphocyte content is markedly higher and the decrease in their concentration is more pronounced; low concentrations of endothelin-1 are associated with a lack of response from the lymphocyte content. The decrease in lymphocyte content in response to general cooling is interrelated with relatively high concentrations of TNF-?, the reaction of which accompanies high concentrations of endothelin-1. Сonclusion. Thus, 20.54% of the examined individuals react to short-term cooling by a decrease in the total lymphocyte count and their activated forms. Redistribution of lymphocytes is associated with an initially high level of endothelin-1 in the blood. At low concentrations of endothelin-1, the reaction to cold from the lymphocytes is not established. Relatively high concentrations of the proinflammatory cytokine reflect the level of activation of the immune background and cause activation of endothelin-1 secretion.
Keywords: cold, endothelin-1, lymphocytes, cytokines
Received 09.04.2018
For citation:
Samodova A.V., Dobrodeeva L.K. Interrelation of the Endothelin-1 Containment in Blood and the Character of Immune Reaction of the Person for Short-Term General Cooling. Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2018, Vol. 15, no. 2, pp. 299–308. DOI: 10.22138/2500-0918-2018-15-2-299-308 (In Russ)
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Authors
Anna V. Samodova
Cand. Sci.(Biol.), Head of the Laboratory of Regulatory Mechanisms of Immunity
annapoletaeva2008@yandex.ru
Liliya K. Dobrodeeva
Dr. Sci. (Med.), Professor, Honored Worker of Science, Chief Researcher of the Laboratory of Regulatory Mechanisms of Immunity, Director
dobrodeevalk@mail.ru
Institute of Physiology of Natural Adaptations of the Federal State Budgetary Institution of Science of the Federal Center for Complex Arctic Studies of the Russian Academy of Sciences. acad. N.P. Laverova
Russian Federation, 163061, Arkhangelsk, Lomonosov Ave., 249
