Molecular genetic surveillance of circulating human nonpoliomyelitis viruses strains: implications for the estimates of epidemic significance and virulence

Ustyuzhanin A. V., Rezaykin A. V., Sergeev A. G., Snitkovskaya T. E.

State Institution of Higher Education “Ural State Medical University”, Healthcare Ministry of the Russian Federation;
Federal State Institution of Science “Yekaterinbug Research Institute of Viral Infections”, Federal’s Inspectorate for Consumers’ Rights and Human Welfare; Federal State Healthcare Institution “Hygiene and Epidemiology Centre in Sverdlovsk region”, Yekaterinburg, Russian Federation

Abstract.
The results of monthly three-year molecular genetic monitoring of nonpoliomyelitis enteroviruses (NPEV) incidence in an indicator group of asymptomatic 3-to-6-years-old virus carriers living in a large industrial centre allowed to reveal a discrepancy between the spikes of NPEV isolation rate from asymptomatic children and the seasonal spikes of enterovirus aseptic meningitis (EVAM) morbidity. Significant difference was observed between the spectra of enteroviral serotypes detected among healthy individuals and the spectra of serotypes detected among EVAM patients. The data obtained indicate that neither the incidence of asymptomatic virus carriers nor the spectrum of NPEV serotypes detected in healthy individuals can be used as a reliable prognostic indicator for epidemic surveillance. The inverse relationship between the proportion of a specific NPEV serotype detected in the CSF samples from EVAM patients and the frequency of its detection among apparently healthy individuals with asymptomatic viral infection from the indicator group provides an estimate for the virulence and epidemic significance of circulating human NPEV strains.

Key words:
circulation of enteroviruses, aseptic meningitis, molecular genetic monitoring

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Authors
Ustyuzhanin Alexandr V.
State Institution of Higher Education “Ural State Medical University”, Healthcare Ministry of the Russian Federation, Yekaterinburg, Russia
Teaching Assistant, Department of Microbiology, Virology and Immunology
3, Repina St., Yekaterinburg, 620028, Russian Federation
e-mail: ust103@yandex.ru

Rezaykin Alexey V.
Federal State Institution of Science “Yekaterinbug Research Institute of Viral Infections”, Russia’s Inspectorate for Consumers’ Rights and Human Welfare, Yekaterinburg, Russia
PhD, Head of Enterovirus Laboratory,
23 Letnyaya St., Yekaterinburg 620030, Yekaterinburg, Russian Federation
e-mail: virus@eniivi.ru

Sergeev Alexandr G.
State Institution of Higher Education “Ural State Medical University”, Healthcare Ministry of the Russian Federation, Yekaterinburg, Russia
PhD, Professor, Head of Microbiology, Virology and Immunology Department
3, Repina St., Yekaterinburg, 620028, Russian Federation
e-mail: sergeev@usma.ru

Snitkovskaya Tatjana E.
Federal State Healthcare Institution “Hygiene and Epidemiology Centre in Sverdlovsk region”, PhD, Head Specialist at the virological laboratory
3 Otdelny Lane, Yekaterinburg, 620078, Russian Federation
e-mail: snitkovskaya_te@66.rospotrebnadzor.ru